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This chapter outlines a protocol for extracting and derivatizing polar metabolites in dried mango (Mangifera indica) for gas chromatography-mass spectrometry (GC-MS) analysis. Due to their high sugar content and complex matrix, dried mangoes present challenges for polar compound profiling, necessitating a robust sample preparation approach. The method differentiates between sugars (e.g., glucose, fructose, sucrose) and non-sugar compounds (e.g., amino acids and organic acids) by employing separate extraction pathways. Cryogenically ground samples are extracted using mixed solvents (methanol:chloroform:water, 5:2:2, v/v/v) with ribitol as an internal standard. Extracts are phase-separated, and aliquots are taken for sugar and non-sugar analysis. Derivatization involves a sequential oximation step using methoxyamine hydrochloride, followed by silylation with MSTFA, enabling improved volatility and thermal stability of analytes. The resulting GC-MS workflow facilitates comprehensive metabolite profiling for nutritional analysis, process monitoring, and product quality control in dried mango and other dehydrated tropical fruits.

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GC-MS-Based Metabolite Profiling Method for Dried Mango

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  • 发表日期：

    2026年

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  • Waqif Agusta

摘要

This chapter outlines a protocol for extracting and derivatizing polar metabolites in dried mango (Mangifera indica) for gas chromatography-mass spectrometry (GC-MS) analysis. Due to their high sugar content and complex matrix, dried mangoes present challenges for polar compound profiling, necessitating a robust sample preparation approach. The method differentiates between sugars (e.g., glucose, fructose, sucrose) and non-sugar compounds (e.g., amino acids and organic acids) by employing separate extraction pathways. Cryogenically ground samples are extracted using mixed solvents (methanol:chloroform:water, 5:2:2, v/v/v) with ribitol as an internal standard. Extracts are phase-separated, and aliquots are taken for sugar and non-sugar analysis. Derivatization involves a sequential oximation step using methoxyamine hydrochloride, followed by silylation with MSTFA, enabling improved volatility and thermal stability of analytes. The resulting GC-MS workflow facilitates comprehensive metabolite profiling for nutritional analysis, process monitoring, and product quality control in dried mango and other dehydrated tropical fruits.