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Antigen presentation is a specialized function of the immune system where peptides generated from cellular or internalized proteins are presented on Major Histocompatibility Complex molecules on the surface of antigen-presenting cells for recognition by T cells. The binding of these MHC-peptide complexes to hypervariable receptors on the surface of a T cell leads to its activation. T cell activation induces increases in cell-surface activation markers such as CD69, which can be tracked by flow cytometry. Here we describe a method that uses flow cytometry and a recombinant T cell to track the presentation of an MHC-II HLA-DR1 restricted epitope generated from the influenza hemagglutinin protein.

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T Cell Assay to Track Presentation of an Influenza Hemagglutinin Epitope Presented by the MHC-II Protein HLA-DR1

  • 实验方案

  • 发表日期：

    2026年

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  • Karthik M. Ramesh,
  • Liying Lu,
  • Lawrence J. Stern

摘要

Antigen presentation is a specialized function of the immune system where peptides generated from cellular or internalized proteins are presented on Major Histocompatibility Complex molecules on the surface of antigen-presenting cells for recognition by T cells. The binding of these MHC-peptide complexes to hypervariable receptors on the surface of a T cell leads to its activation. T cell activation induces increases in cell-surface activation markers such as CD69, which can be tracked by flow cytometry. Here we describe a method that uses flow cytometry and a recombinant T cell to track the presentation of an MHC-II HLA-DR1 restricted epitope generated from the influenza hemagglutinin protein.