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The protocol described in this chapter focuses on the detection of foodborne viruses, specifically human norovirus and hepatitis A virus in berries. Viruses are eluted from the berries and precipitated using PEG/NaCl. Chloroform-butanol is also used for purification. RNA extraction is based on disruption of the viral capsid using a chaotropic lysis buffer and silica beads to recover the RNA. RT-qPCR is used to detect the viral genome.

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Detection of Human Norovirus and Hepatitis A Virus in Berries

  • 章节

  • 发表日期：

    2026年

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  • Éric Jubinville,
  • Marie-Ève Collard,
  • Anne-Marie Lauzier,
  • Julie Jean

摘要

The protocol described in this chapter focuses on the detection of foodborne viruses, specifically human norovirus and hepatitis A virus in berries. Viruses are eluted from the berries and precipitated using PEG/NaCl. Chloroform-butanol is also used for purification. RNA extraction is based on disruption of the viral capsid using a chaotropic lysis buffer and silica beads to recover the RNA. RT-qPCR is used to detect the viral genome.