Salmonellosis is one of the major foodborne diseases. Particularly Salmonella enterica and its serovars Enteritidis and Typhimurium, are significant pathogens causing nontyphoidal salmonellosis in humans, often transmitted through contaminated food products. Timely detection of these pathogens is crucial for food safety and public health. This chapter describes a multiplex PCR (mPCR) method for the rapid detection of Salmonella Enteritidis and Typhimurium in meat products. The method involves pre-enrichment of samples in buffered peptone water, DNA extraction using the phenol-chloroform method, and PCR amplification employing a primer set targeting the srfC gene specific to Salmonella enterica, along with two additional primer sets, STM4495 and SEN1392, targeting S. Typhimurium and S. Enteritidis, respectively. The mPCR reaction is optimized to detect as low as 23 CFU/PCR for S. Typhimurium and 22 CFU/PCR for S. Enteritidis, offering a rapid and reliable diagnostic tool for foodborne pathogen surveillance. The effectiveness of this method is demonstrated through gel electrophoresis, producing distinct DNA fragments for each target. This approach provides a sensitive, specific, and efficient alternative to traditional culture-based detection methods, supporting enhanced food safety monitoring and public health initiatives.