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Mass spectrometry (MS) is the analytical technique of choice when information about molecular mass and structural characteristics of minute sample quantities are desired. Here, we report a generally applicable, robust, and fast method for sample preparation and matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF) analysis of D-dopachrome tautomerase (D-DT or MIF-2) in the presence of a small molecule ligand. We show that noncovalent protein–ligand complexes can be detected. Our method will allow researchers to quickly screen large libraries of ligands against a given protein target and should be considered a supplement to other detection techniques for noncovalent protein–ligand complexes.

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Detection of Noncovalent Protein–Ligand Complexes of D-DT Variants by MALDI-TOF Mass Spectrometry

  • 实验方案

  • 发表日期：

    2026年

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  • Andrew Parkins,
  • Georgios Pantouris,
  • Andreas H. Franz

摘要

Mass spectrometry (MS) is the analytical technique of choice when information about molecular mass and structural characteristics of minute sample quantities are desired. Here, we report a generally applicable, robust, and fast method for sample preparation and matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF) analysis of D-dopachrome tautomerase (D-DT or MIF-2) in the presence of a small molecule ligand. We show that noncovalent protein–ligand complexes can be detected. Our method will allow researchers to quickly screen large libraries of ligands against a given protein target and should be considered a supplement to other detection techniques for noncovalent protein–ligand complexes.