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In vitro co-culture models of human cells and microbial pathogens offer a controlled system to study host–pathogen interactions. This chapter details the methods to establish such co-culture models for a fungal and an amoebic infection using two different human cell lines. Employing this experimental system allows the researcher to evaluate MIF’s contribution to infection-driven host cell death and pro-inflammatory gene expression.

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Modeling Fungal and Amoebic Infections: Co-culture Approaches to Study MIF’s Role in Infection-Induced Inflammatory Responses

  • 实验方案

  • 发表日期：

    2026年

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  • Swagata Ghosh,
  • Mathan L

摘要

In vitro co-culture models of human cells and microbial pathogens offer a controlled system to study host–pathogen interactions. This chapter details the methods to establish such co-culture models for a fungal and an amoebic infection using two different human cell lines. Employing this experimental system allows the researcher to evaluate MIF’s contribution to infection-driven host cell death and pro-inflammatory gene expression.