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The isolation of bone marrow-derived macrophages (BMDMs) from the bones of mice provides an excellent tool to study the different activation states of macrophages both in vitro and ex vivo. This chapter utilizes BMDMs derived from wild-type and -794 CATT7 human transgenic mice to investigate the influence of high human MIF expression on macrophage polarization. Real-time qPCR and ELISA can be used to easily distinguish M1 and M2 polarization phenotypes.

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Polarization of Bone Marrow-Derived Macrophages from Wild-Type and High Human MIF-Expressing CATT7 Polymorphic Mice

  • 实验方案

  • 发表日期：

    2026年

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  • Molly Dunlop,
  • Sinéad Ryan,
  • Ian J. Hawthorne,
  • Michelle E. Armstrong,
  • Seamas C. Donnelly,
  • Karen English

摘要

The isolation of bone marrow-derived macrophages (BMDMs) from the bones of mice provides an excellent tool to study the different activation states of macrophages both in vitro and ex vivo. This chapter utilizes BMDMs derived from wild-type and -794 CATT7 human transgenic mice to investigate the influence of high human MIF expression on macrophage polarization. Real-time qPCR and ELISA can be used to easily distinguish M1 and M2 polarization phenotypes.