Recombinant antibodies and novel display technologies have significantly advanced the development of antibodies. However, new techniques are still required to select rare hapten-specific clones from large recombinant libraries. In this chapter, we describe a novel selection procedure for the identification and subsequent isolation of rare, recombinant antibodies from a relatively large immune library (∼ 4.4 × 106). This technique, which combined preselection of an immune phage library with yeast display and competitive FACS, yielded high percentages (20%−40%) of hapten-specific scFvs in the final pool of selected cells.