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To decipher the complex tissue microenvironment for comprehensively elucidating the biological machinery, spatial proteomics is becoming popular. Laser microdissection (LMD) technology combined with integrated sample preparation and high-sensitive liquid chromatography-mass spectrometry (LC-MS) is the most powerful strategy. In this chapter, we describe the protocols for tissue slice preparation and staining, LMD-based histological regions collection, simple and integrated spintip-based proteomics technology (SISPROT) based sample preparation, and highly sensitive LC-MS analysis.

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Fully Integrated Sample Preparation-Based Spatial Proteomic Analysis

  • 实验方案

  • 发表日期：

    2026年

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  • Qian Kong,
  • Xi Wang,
  • Ruijun Tian

摘要

To decipher the complex tissue microenvironment for comprehensively elucidating the biological machinery, spatial proteomics is becoming popular. Laser microdissection (LMD) technology combined with integrated sample preparation and high-sensitive liquid chromatography-mass spectrometry (LC-MS) is the most powerful strategy. In this chapter, we describe the protocols for tissue slice preparation and staining, LMD-based histological regions collection, simple and integrated spintip-based proteomics technology (SISPROT) based sample preparation, and highly sensitive LC-MS analysis.