Scholarlink

Chemotherapeutic agents exert antineoplastic effects by damaging the DNA of cancer cells, leading to cell death. However, this effect is not specific, and may affect healthy cells and cause several adverse events such as myelosuppression and gastrointestinal mucositis. The term gastrointestinal mucositis refers to lesions in the mucosa, which include the oral cavity, pharynx, larynx, stomach, and intestine. The focus of this chapter will be the chemotherapy-induced intestinal damage, which can lead to weight loss, diarrhea, constipation, and increased predisposition to infections. Despite its severity, there is no treatment available for mucositis, and the pathophysiology of the disease has not been fully elucidated so far. This highlights the relevance of studying the mechanism of the disease and possible intervention strategies in intestinal mucositis. Here, we provide a detailed description of the main methodologies involved in the evaluation of changes induced by chemotherapy in a murine model, namely, 5-Fluorouracil (5-FU)-induced mucositis. These parameters include (i) assessment of body weight, (ii) stool consistency, (iii) fecal occult blood, and (iv) intestinal permeability. In addition, we describe a protocol to model opportunistic infection after chemotherapy, by exposing mice treated with 5-FU to Candida albicans in the drinking water.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Modeling Gut Barrier Dysfunction and Subsequent Opportunistic Infection by Candida albicans During Chemotherapy-Induced Intestinal Mucositis in Mice

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Rafaela Ribeiro Alvares Batista,
  • Renan Vasconcelos da Graça-Filho,
  • Giuliana do Nascimento Ozores,
  • Ana Caroline de Lima,
  • Caio Tavares Fagundes

摘要

Chemotherapeutic agents exert antineoplastic effects by damaging the DNA of cancer cells, leading to cell death. However, this effect is not specific, and may affect healthy cells and cause several adverse events such as myelosuppression and gastrointestinal mucositis. The term gastrointestinal mucositis refers to lesions in the mucosa, which include the oral cavity, pharynx, larynx, stomach, and intestine. The focus of this chapter will be the chemotherapy-induced intestinal damage, which can lead to weight loss, diarrhea, constipation, and increased predisposition to infections. Despite its severity, there is no treatment available for mucositis, and the pathophysiology of the disease has not been fully elucidated so far. This highlights the relevance of studying the mechanism of the disease and possible intervention strategies in intestinal mucositis. Here, we provide a detailed description of the main methodologies involved in the evaluation of changes induced by chemotherapy in a murine model, namely, 5-Fluorouracil (5-FU)-induced mucositis. These parameters include (i) assessment of body weight, (ii) stool consistency, (iii) fecal occult blood, and (iv) intestinal permeability. In addition, we describe a protocol to model opportunistic infection after chemotherapy, by exposing mice treated with 5-FU to Candida albicans in the drinking water.