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This chapter describes the preparation of metagenomic DNA samples to produce long-read Oxford Nanopore-compatible libraries for shotgun whole-genome sequencing (WGS). This is of value for projects needing to assemble novel unculturable bacterial species and where higher resolution is required.

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Long-Read Metagenomic Sequencing

  • 实验方案

  • 出版模式：

    开放获取

  • 发表日期：

    2026年

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  • Dave Baker,
  • Cara-Jane Moss

摘要

This chapter describes the preparation of metagenomic DNA samples to produce long-read Oxford Nanopore-compatible libraries for shotgun whole-genome sequencing (WGS). This is of value for projects needing to assemble novel unculturable bacterial species and where higher resolution is required.