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This chapter describes the targeting of the prokaryotic 16S ribosomal RNA gene (16S rRNA) using both short-read Illumina Sequencing and long-read Nanopore Sequencing. 16S rRNA targets can be present in multiple copies across the genome and are another consideration when trying to quantitatively determine the proportion of different bacterial species present in samples of different origin, including the gut and feces.

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16S rRNA Library Preparation and Sequencing

  • 实验方案

  • 出版模式：

    开放获取

  • 发表日期：

    2026年

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  • Dave Baker,
  • Cara–Jane Moss

摘要

This chapter describes the targeting of the prokaryotic 16S ribosomal RNA gene (16S rRNA) using both short-read Illumina Sequencing and long-read Nanopore Sequencing. 16S rRNA targets can be present in multiple copies across the genome and are another consideration when trying to quantitatively determine the proportion of different bacterial species present in samples of different origin, including the gut and feces.