Viruses are omnipresent and the most abundant biological entities on the planet. They play a critical role in shaping the ecology and evolution of all life forms. This chapter provides methods to perform viromics or viral metagenomics, starting with the creation of a mock community to control experimental variables, followed by methods for nucleic acid extraction, and finally bioinformatics workflows for the processing and analysis of the sequence data.

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Virome Analysis

  • Revathy Krishnamurthi,
  • Rik Haagmans,
  • Ryan Cook,
  • Alise J. Ponsero,
  • Evelien M. Adriaenssens

摘要

Viruses are omnipresent and the most abundant biological entities on the planet. They play a critical role in shaping the ecology and evolution of all life forms. This chapter provides methods to perform viromics or viral metagenomics, starting with the creation of a mock community to control experimental variables, followed by methods for nucleic acid extraction, and finally bioinformatics workflows for the processing and analysis of the sequence data.