The understanding that cellular metabolism underlines the differentiation, activation, and function of immune cells has opened new avenues to modulate immunity. Here, we describe a method to analyze cellular metabolism using the quintessential immunological technique: flow cytometry. We analyze single cell suspensions, by combining the staining of surface immune cell markers and nutrient transporters, with the staining using metabolic dyes, to readout surrogates of metabolic pathway utilization with cell subset resolution.