Medicinal mushrooms are categorized as beneficial fungi and are used for their restorative attributes. The form of bioactive additives discovered in those mushrooms, together with proteins, flavonoids, phenolics, terpenoids, polysaccharides, and sterols, has made them a focal point of contemporary clinical studies. They are famous for their dietary advantages; however, additionally, they have some medicinal qualities, which include antimicrobial, anticancer, and, maximum notably, antifungal effects. Recent studies have verified the antifungal properties of diverse mushrooms, together with Cordyceps sinensis, Ganoderma lucidum, and Lentinula edodes, which comprise compounds that include polysaccharides, triterpenoids, and β-glucans, which may effectively inhibit pathogenic fungi. The preparation of mushroom samples and the usage of solvent-primarily based totally extraction and purification strategies are the primary steps in the extraction process of those bioactive substances. The antifungal activity of these extracts can be evaluated using assays such as the poisoned food technique, which helps in identifying natural antifungal agents for potential therapeutic applications.