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Fungal diversity plays an essential role in the food industry by diversifying gastronomy and enabling the development of new flavors and products through fermentation. Genomic studies can be used to characterize new fungal strains and map their genetic variations. Moreover, fungi express different genes when confronted with different substrates, which has a direct influence the organoleptic properties of fermented products. This chapter discusses methods for extracting DNA from fungi, sequencing, and analyzing its genetic variations. In addition, it presents a detailed transcriptomic workflow, including RNA extraction, sequencing, and subsequent analysis with bioinformatics methods is described. Platforms such as Galaxy and R-based environments like Bioconductor will be explored to identify differentially expressed genes and their biological function.

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Fungal Characterization and Identification: Genomic and Transcriptomic Approaches

  • 章节

  • 发表日期：

    2026年

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  • Pablo Navarro Simarro,
  • Alberto José López-Jiménez

摘要

Fungal diversity plays an essential role in the food industry by diversifying gastronomy and enabling the development of new flavors and products through fermentation. Genomic studies can be used to characterize new fungal strains and map their genetic variations. Moreover, fungi express different genes when confronted with different substrates, which has a direct influence the organoleptic properties of fermented products. This chapter discusses methods for extracting DNA from fungi, sequencing, and analyzing its genetic variations. In addition, it presents a detailed transcriptomic workflow, including RNA extraction, sequencing, and subsequent analysis with bioinformatics methods is described. Platforms such as Galaxy and R-based environments like Bioconductor will be explored to identify differentially expressed genes and their biological function.