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Morphological profiling with the Cell Painting assay started a new era in phenotypic screening approaches by harnessing comprehensive morphological profiles of cellular perturbations. This enables an unbiased characterization of the effects of small chemical compounds. We established and extensively validated a Cell Painting protocol to screen the compound libraries of the European initiative EU-OPENSCREEN ( www.eu-openscreen.eu ) in order to find robust and reproducible links between the known target or the pathway mechanism of each compound to the phenotypic profile. In this chapter, we describe the Cell Painting procedure, image analysis, and the downstream data processing procedures.

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Cell Painting Protocol to Characterize Morphological Profiles of Large Compound Collections Using the EU-OPENSCREEN Library

  • 实验方案

  • 出版模式：

    开放获取

  • 发表日期：

    2026年

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  • Christopher Wolff,
  • Martin Neuenschwander,
  • Michelle Müller,
  • Astrid Mühl,
  • Jens Peter von Kries,
  • Christopher Schmied,
  • Edgar Specker

摘要

Morphological profiling with the Cell Painting assay started a new era in phenotypic screening approaches by harnessing comprehensive morphological profiles of cellular perturbations. This enables an unbiased characterization of the effects of small chemical compounds. We established and extensively validated a Cell Painting protocol to screen the compound libraries of the European initiative EU-OPENSCREEN ( www.eu-openscreen.eu ) in order to find robust and reproducible links between the known target or the pathway mechanism of each compound to the phenotypic profile. In this chapter, we describe the Cell Painting procedure, image analysis, and the downstream data processing procedures.