Salinity is one of the severe abiotic stress factors that affect approximately 50% of planted and irrigated agricultural fields. The determination of plant response to salinity is the key factor that reduces yield lost in crop production. Transgenerational plasticity is a nongenetic modification that provides a mechanism for plants to “remember” and adapt to environmental conditions experienced by their ancestors, contributing to the phenotypic plasticity and resilience of plant populations over successive generations. This ability to retain stress memory enables plants to respond more effectively to recurring salinity stress, potentially enhancing crop adaptation and productivity. The determination of transgenerational plasticity of salinity tolerance has some key research elements that can be focused on heritable modifications. In this chapter, we explain the methods of transgenerational plasticity of salinity tolerance research.