CUT&Tag (Cleavage Under Targets and Tagmentation) is a powerful method for chromatin profiling based on an enzyme-tethering strategy. Compared with chromatin immunoprecipitation (ChIP) assay, CUT&Tag requires smaller amount of cell/nuclei input and generates data with higher signal-to-noise ratio, which has been widely applied in profiling histone modifications and protein-DNA interactions. Here, we describe a detailed protocol for bulk-cell CUT&Tag in plants and briefly introduced other derivative CUT&Tag methods.