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This protocol describes step-by-step how to discover and annotate DNA motifs in regulatory regions of clusters/modules of co-expressed genes in plants, including non-model species. It also explains how to control the significance of the results and how to associate the discovered motifs with potentially binding transcription factors (TFs). These tasks can all be done in a web browser. While it uses the plant-dedicated mirror of the Regulatory Sequence Analysis Tools (RSAT, http://plants.rsat.eu ), it can be used on any organism hosted at any RSAT mirror.

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Web-Based Discovery of Regulatory Motifs in Non-model Plants

  • 实验方案

  • 发表日期：

    2026年

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  • Najla Ksouri,
  • Ammar Abdalrahem,
  • Francesc Montardit-Tarda,
  • Jaime A. Castro-Mondragón,
  • Jacques van Helden,
  • Yolanda Gogorcena,
  • Bruno Contreras-Moreira

摘要

This protocol describes step-by-step how to discover and annotate DNA motifs in regulatory regions of clusters/modules of co-expressed genes in plants, including non-model species. It also explains how to control the significance of the results and how to associate the discovered motifs with potentially binding transcription factors (TFs). These tasks can all be done in a web browser. While it uses the plant-dedicated mirror of the Regulatory Sequence Analysis Tools (RSAT, http://plants.rsat.eu ), it can be used on any organism hosted at any RSAT mirror.