Extracellular vesicles (EVs) play a key role in many physiological and pathophysiological processes and hold both therapeutic and diagnostic promise. However, the broad definition of storage conditions to ensure EV stability remains unclear. In recent years, we and others have explored different concepts and defined protocols aiming to stabilize EVs and ensure reproducible EV recovery. Despite these advances, the assessment of EV stability remains a challenge due to the multimodal composition of EVs and the variety of their biological functions. Here, we summarize and discuss the current state-of-the-art and describe selected experimental procedures that have been utilized to stabilize EVs and to assess their stability and recovery, with a focus on cell-culture-derived EVs.