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ImmunotherapiesImmunotherapies blocking immune checkpoint PD-1/PD-L1 signaling have shown great promise in a variety of cancers, but most patients fail to respond or develop resistance. One of the factors determining the responsiveness to PD-1/PD-L1 targeting immunotherapies is surface expression of PD-L1 on cancer cells. Here we describe a flow cytometry protocol for the analysis of cell surface expression of PD-L1 induced by IFNγ in ovarian cancer cells, by using antibody that specifically recognizes the extracellular domain of PD-L1.

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Flow Cytometry Analysis of IFNγ-Induced PD-L1 Cell Surface Expression in Ovarian Cancer Cells

  • 实验方案

  • 发表日期：

    2026年

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  • Sveta Padmanabhan,
  • Yue Zou,
  • Ivana Vancurova

摘要

ImmunotherapiesImmunotherapies blocking immune checkpoint PD-1/PD-L1 signaling have shown great promise in a variety of cancers, but most patients fail to respond or develop resistance. One of the factors determining the responsiveness to PD-1/PD-L1 targeting immunotherapies is surface expression of PD-L1 on cancer cells. Here we describe a flow cytometry protocol for the analysis of cell surface expression of PD-L1 induced by IFNγ in ovarian cancer cells, by using antibody that specifically recognizes the extracellular domain of PD-L1.