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Combining immunoassays with nucleic acid-based amplification and detection, the proximity ligation assay (PLA) is a useful tool for immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein–protein interactions in fixed cells and tissue samples. Here, we present a detailed procedure for detecting protein–protein interactions in cancer cells with a commercially available Naveni® in situ proximity ligation technology to assist the researcher in successfully performing the experiments.

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Proximity Ligation Assay to Detect Protein-Protein Interaction in Cancer Cells

  • 实验方案

  • 发表日期：

    2026年

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  • Patricia Rozario,
  • Yan Zhu

摘要

Combining immunoassays with nucleic acid-based amplification and detection, the proximity ligation assay (PLA) is a useful tool for immunofluorescent detection, visualization, and quantification of individual proteins, protein modifications, and protein–protein interactions in fixed cells and tissue samples. Here, we present a detailed procedure for detecting protein–protein interactions in cancer cells with a commercially available Naveni® in situ proximity ligation technology to assist the researcher in successfully performing the experiments.