Ferroptosis is a unique form of regulated cell death characterized by the toxic buildup of lipid peroxides in plasma membranes. Uncontrolled ferroptosis has been linked to various pathological conditions, including cancer progression, neurodegeneration, kidney damage, ischemia/reperfusion injury, and T-cell immunity. In this article, we present a method for detecting ferroptosis by measuring lipid peroxides in cellular membranes with the Liperfluo and BODIPY-C11 probes. The potential role of ferroptosis in immune-modulatory cells can also be assessed using this approach.