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Tracking, isolating, and studying antigen-specific B cells is challenging due to their low frequencies. Here, I describe a protocol to study enriched Trypanosoma cruzi (T. cruzi)-specific B cells by flow cytometry. Compared to alternative strategies (e.g., ELISA and ELISPOT), this approach allows detailed phenotyping and/or cell sorting for downstream analysis of T. cruzi-specific B cells.

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The Use of B-cell Tetramers for the Study of Trypanosoma cruzi-Specific B cells

  • 实验方案

  • 发表日期：

    2026年

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  • Damián Pérez-Mazliah

摘要

Tracking, isolating, and studying antigen-specific B cells is challenging due to their low frequencies. Here, I describe a protocol to study enriched Trypanosoma cruzi (T. cruzi)-specific B cells by flow cytometry. Compared to alternative strategies (e.g., ELISA and ELISPOT), this approach allows detailed phenotyping and/or cell sorting for downstream analysis of T. cruzi-specific B cells.