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An effective method to quantify parasite loads is key to evaluating anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening of drugs against Cryptosporidium parvum was previously infeasible using labor-intensive traditional assays. Here, we describe a simplified quantitative RT-PCR assay that directly uses cell lysates as templates, enabling high-throughput quantification of parasite loads. This assay can also be used to evaluate drug efficacy against the growth of C. parvum in vitro.

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Phenotypic High-Throughput Screening and Drug Efficacy Test Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR

  • 实验方案

  • 发表日期：

    2026年

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  • Chenchen Wang,
  • Dongqiang Wang,
  • Haili Zhang,
  • Guan Zhu

摘要

An effective method to quantify parasite loads is key to evaluating anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening of drugs against Cryptosporidium parvum was previously infeasible using labor-intensive traditional assays. Here, we describe a simplified quantitative RT-PCR assay that directly uses cell lysates as templates, enabling high-throughput quantification of parasite loads. This assay can also be used to evaluate drug efficacy against the growth of C. parvum in vitro.