Microelectrode arrays offer an exciting opportunity to probe and characterize the functional status of dystrophin-deficient muscle tissue vis-à-vis patient-specific iPSC-derived skeletal muscle (SkM) and cardiac muscle (CM). Here, we present a method to culture and analyze baseline electrophysiological profiles of Duchenne muscular dystrophy (DMD) iPSC-SkM and iPSC-CM using MEA dishes.

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Culture and Electrophysiological Analysis of Patient-Specific iPSCs Using Microelectrode Array Dishes

  • Bayardo I. Garay,
  • Rita C. R. Perlingeiro

摘要

Microelectrode arrays offer an exciting opportunity to probe and characterize the functional status of dystrophin-deficient muscle tissue vis-à-vis patient-specific iPSC-derived skeletal muscle (SkM) and cardiac muscle (CM). Here, we present a method to culture and analyze baseline electrophysiological profiles of Duchenne muscular dystrophy (DMD) iPSC-SkM and iPSC-CM using MEA dishes.