Scholarlink

The induced pluripotent stem cell (iPSC) technology provides human neural cells unprecedented material for neuroscience research. Lentiviral vectors have the advantage of being able to transfer genes into nondividing cells, which is useful for the identification and purification of specific neurons generated from human iPSCs, as well as for analysis of their function. Furthermore, the lentiviral vector has long-term expression and large gene capacity. This chapter describes protocols for lentiviral generation, labeling of iPSC-derived motor neurons with fluorescent proteins, and imaging of synaptic function.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Lentiviral Vector-Mediated Gene Delivery for iPSC-Related Neuroscience Research

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Taro Okunomiya,
  • Keiko Imamura,
  • Taku Kashiyama,
  • Takashi Sakurai,
  • Hiroyuki Hioki,
  • Haruhisa Inoue

摘要

The induced pluripotent stem cell (iPSC) technology provides human neural cells unprecedented material for neuroscience research. Lentiviral vectors have the advantage of being able to transfer genes into nondividing cells, which is useful for the identification and purification of specific neurons generated from human iPSCs, as well as for analysis of their function. Furthermore, the lentiviral vector has long-term expression and large gene capacity. This chapter describes protocols for lentiviral generation, labeling of iPSC-derived motor neurons with fluorescent proteins, and imaging of synaptic function.