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Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein–ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP–protein interactions using a Creoptix WAVEsystem.

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The Use of Grating-Coupled Interferometry to Study Inositol Pyrophosphate–Protein Interactions

  • 实验方案

  • 发表日期：

    2025年

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  • Kristina Sturm,
  • Michael Hothorn

摘要

Grating-coupled interferometry (GCI) is a biophysical method suitable to studying the binding kinetics of protein–ligand interactions. Here, we describe the application of biotinylated inositol pyrophosphate (PP-InsP) nutrient messengers to characterize PP-InsP–protein interactions using a Creoptix WAVEsystem.