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Phosphorylation is a key regulatory mechanism of cellular functions, and a majority of known bacteriophytochromes (BphPs) act as histidine kinases (HKs) in two-component signaling. These red/far-red light-sensing HKs play a role in diverse light responses in bacteria. In this chapter, we introduce protocols to easily track the net kinase and phosphatase activities of BphP HKs. The protocols provided here apply Phos-tag™ acrylamide gel electrophoresis for protein samples under controlled illumination conditions. They can be applied to BphP HKs and with a few modifications to photoreceptor HKs in general.

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Detection of Histidine Kinase Activities of Bacteriophytochromes with Phos-tag™ Acrylamide Gel Electrophoresis

  • 实验方案

  • 发表日期：

    2026年

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  • Iida Tuure,
  • Heikki Takala

摘要

Phosphorylation is a key regulatory mechanism of cellular functions, and a majority of known bacteriophytochromes (BphPs) act as histidine kinases (HKs) in two-component signaling. These red/far-red light-sensing HKs play a role in diverse light responses in bacteria. In this chapter, we introduce protocols to easily track the net kinase and phosphatase activities of BphP HKs. The protocols provided here apply Phos-tag™ acrylamide gel electrophoresis for protein samples under controlled illumination conditions. They can be applied to BphP HKs and with a few modifications to photoreceptor HKs in general.