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The linear tetrapyrrole-binding cyanobacteriochrome (CBCR) photoreceptors have been extensively analyzed so far and found to be highly diverse in spectral properties. Here, we describe the methods to search novel CBCR molecules from the database, perform site-saturation random mutagenesis and colony-based protein expression, and express the CBCR molecules with various chromophore synthetic systems. These methods will contribute to further discovery and engineering of the CBCR molecules.

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Discovery and Engineering of Unique Cyanobacteriochrome GAF Domains

  • 实验方案

  • 发表日期：

    2026年

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  • Takahisa Suzuki,
  • Ayano Yoneda,
  • Tomo Yokota,
  • Hiroki Hoshino,
  • Keita Miyake,
  • Keiji Fushimi,
  • Rei Narikawa

摘要

The linear tetrapyrrole-binding cyanobacteriochrome (CBCR) photoreceptors have been extensively analyzed so far and found to be highly diverse in spectral properties. Here, we describe the methods to search novel CBCR molecules from the database, perform site-saturation random mutagenesis and colony-based protein expression, and express the CBCR molecules with various chromophore synthetic systems. These methods will contribute to further discovery and engineering of the CBCR molecules.