Scholarlink

Signal peptides are essential for the secretion of recombinant proteins using Komagataella phaffii. The N-terminal signal peptide is removed by proteases during the translocation process. However, this processing is not always complete, resulting in an N-terminal extension which is problematic for some proteins. In this chapter, we present a straightforward approach to evaluate signal peptide processing by a high-throughput solid-phase agar plate assay.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Lytic Polysaccharide Monooxygenases as Reporters for Signal Peptide Evaluation in Pichia pastoris

  • 实验方案

  • 发表日期：

    2026年

查看全文
  • Andrea K. Hönikl,
  • Lukas Rieder

摘要

Signal peptides are essential for the secretion of recombinant proteins using Komagataella phaffii. The N-terminal signal peptide is removed by proteases during the translocation process. However, this processing is not always complete, resulting in an N-terminal extension which is problematic for some proteins. In this chapter, we present a straightforward approach to evaluate signal peptide processing by a high-throughput solid-phase agar plate assay.