The microbial loop is a central system in the rhizosphere, where most microbes compete, and their various activities establish a distinct soil microflora. These microorganisms include bacteria, fungi, protozoa, viruses, and mold which may produce antibiotics, exopolysaccharide (EPS), and organic acid or act as bacterivorous organisms. Endosymbionts are organisms that live inside a host in a symbiotic relationship without causing harm. The biologically active compounds or metabolites produced by endosymbionts benefit the host. EPSs are high molecular weight polymeric molecules composed of sugar residues. The characteristic properties of EPS such as high water holding capacity, protection against biotic and abiotic stress, and nutrient acquisition benefit both the producing endosymbionts and their host. The ability of endosymbionts to produce EPS enhances their symbiotic association with the host. EPS producers thus play an important role in soil fertility and crop sustainability. The present protocol briefly describes methods for screening EPS-producing endosymbionts.