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RNA sequencing (RNA-Seq) is a next-generation sequencing method, routinely used in laboratories to detect abnormal transcripts with oncogenic activity after RNA extraction from formalin-fixed-paraffin-embedded (FFPE) samples of non-small-cell-lung cancers (NSCLC), then allowing targeting of the corresponding fusion kinase with adapted pharmacologic compounds in the clinical practice. Here, we provide a detailed protocol for total nucleic acid (i.e., RNA and DNA) extraction from FFPE samples and targeted RNA-Seq, by the FusionPlex® method which allows the detection of the most current fusion transcripts found in NSCLC.

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A Wet Lab Procedure for Gene Fusion Analysis by Targeted RNA Next-Generation Sequencing in Lung Cancer

  • 实验方案

  • 发表日期：

    2025年

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  • Sylvain Lacorre,
  • Maéva Chaumet,
  • Murat Sahin,
  • Emilie Guérin,
  • Paco Derouault,
  • Léa Darnaud,
  • Alexandre Perani,
  • Karine Durand

摘要

RNA sequencing (RNA-Seq) is a next-generation sequencing method, routinely used in laboratories to detect abnormal transcripts with oncogenic activity after RNA extraction from formalin-fixed-paraffin-embedded (FFPE) samples of non-small-cell-lung cancers (NSCLC), then allowing targeting of the corresponding fusion kinase with adapted pharmacologic compounds in the clinical practice. Here, we provide a detailed protocol for total nucleic acid (i.e., RNA and DNA) extraction from FFPE samples and targeted RNA-Seq, by the FusionPlex® method which allows the detection of the most current fusion transcripts found in NSCLC.