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The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions. We present the full protocol of water sequencing from sample collection to library preparation.

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Targeted 16S rRNA Gene Sequencing for Water Samples

  • 实验方案

  • 发表日期：

    2025年

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  • Mélanie Pimenta,
  • Margaux Gaschet,
  • Sylvain Meyer

摘要

The choice of variable region to amplify in 16S rRNA-targeted amplicon sequencing has long been a matter of debate. Here, we describe a method for sequencing multiple variable regions with the Ion 16S Metagenomics kit, which amplifies six different amplicons covering seven 16S variable regions. We present the full protocol of water sequencing from sample collection to library preparation.