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Ligation-mediated RT-PCR is a rapid and highly sensitive solution to detect the presence of gene fusions in cancers. Here, we provide a detailed protocol which allows the detection of hundreds of these tumor markers in a single assay, in a rapid turnaround time.

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Detection of Cancer-Associated Gene Fusion by Ligation-Dependent RT-PCR

  • 实验方案

  • 发表日期：

    2025年

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  • Vinciane Rainville,
  • Marie-Delphine Lanic,
  • Fanny Drieux,
  • Nicolas Piton,
  • David Rizzo,
  • Fabrice Jardin,
  • Philippe Ruminy

摘要

Ligation-mediated RT-PCR is a rapid and highly sensitive solution to detect the presence of gene fusions in cancers. Here, we provide a detailed protocol which allows the detection of hundreds of these tumor markers in a single assay, in a rapid turnaround time.