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Many nucleoporins interact with the pore membrane, often via amphipathic helices. Liposome floatation assays are convenient and flexible assays to analyze these protein-membrane interactions and their dependence on specific lipid composition and membrane curvature. We outline here our protocols for the generation of different-sized small unilamellar liposomes of defined lipid compositions and describe how these are employed in floatation assays.

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Liposome Floatation Assays to Study Membrane Interactions of Nucleoporins

  • 实验方案

  • 发表日期：

    2025年

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  • Marianna Tatarek-Nossol,
  • Wolfram Antonin

摘要

Many nucleoporins interact with the pore membrane, often via amphipathic helices. Liposome floatation assays are convenient and flexible assays to analyze these protein-membrane interactions and their dependence on specific lipid composition and membrane curvature. We outline here our protocols for the generation of different-sized small unilamellar liposomes of defined lipid compositions and describe how these are employed in floatation assays.