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Isolation of cells from tissues can be as daunting a task as it is satisfying. Primary cells closely resemble the tissue they originate from, recapitulating its properties. Here, the procedure for isolating smooth muscle cells (SMCs) from the rat aorta using enzymatic digestions is outlined. Notwithstanding the difference in size and thickness of the aortic wall, the protocol can also be applied to isolate cells from the mouse aorta, which is advantageous considering the plethora of transgenic mice models. A single-cell suspension of cells staining positive for smooth muscle α-actin is obtained.

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Isolation of Rodent Aortic Smooth Muscle Cells by Enzymatic Digestions

  • 实验方案

  • 发表日期：

    2025年

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  • Evangelia Pantazaka

摘要

Isolation of cells from tissues can be as daunting a task as it is satisfying. Primary cells closely resemble the tissue they originate from, recapitulating its properties. Here, the procedure for isolating smooth muscle cells (SMCs) from the rat aorta using enzymatic digestions is outlined. Notwithstanding the difference in size and thickness of the aortic wall, the protocol can also be applied to isolate cells from the mouse aorta, which is advantageous considering the plethora of transgenic mice models. A single-cell suspension of cells staining positive for smooth muscle α-actin is obtained.