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Circulating endothelial cells (CECs) are a key indicator of cardiovascular dysregulation. It is possible to efficiently isolate live CECs from patient blood samples for subsequent analysis by using microfluidic systems. Antibody-based methods ensure efficient and high-accuracy isolation of CECs. By performing this isolation under septic conditions, it is possible to collect viable CECs, which can then be cultured for further study. In this work, we detail the process of isolating and enumerating CECs from patient blood using biotinylated x-hu anti-CD31 antibody-coated staggered herringbone (μ-HB) devices.

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Isolation and Enumeration of Circulating Endothelial Cells with Antibody-Labeled Staggered Herringbone Microfluidic System

  • 实验方案

  • 发表日期：

    2025年

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  • Md Mahedi Hasan,
  • Angel Ricardo Lerma,
  • Taslim A. Al-Hilal

摘要

Circulating endothelial cells (CECs) are a key indicator of cardiovascular dysregulation. It is possible to efficiently isolate live CECs from patient blood samples for subsequent analysis by using microfluidic systems. Antibody-based methods ensure efficient and high-accuracy isolation of CECs. By performing this isolation under septic conditions, it is possible to collect viable CECs, which can then be cultured for further study. In this work, we detail the process of isolating and enumerating CECs from patient blood using biotinylated x-hu anti-CD31 antibody-coated staggered herringbone (μ-HB) devices.