Scholarlink

Protein–protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein networks in bacteria. In this chapter, we outline a standardized protocol for implementing miniTurbo-dependent proximity labeling in bacterial models. This approach enables the unbiased identification of interacting and proximal proteins under native conditions, providing valuable insights into bacterial cell physiology.

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Identification of In Vivo Protein Networks Using miniTurbo Proximity Labeling in Bacteria

  • 实验方案

  • 发表日期：

    2025年

查看全文
  • Coralie Tesseur,
  • Ophélie Remy,
  • Géraldine Laloux,
  • Yoann Santin

摘要

Protein–protein interactions play a crucial role in many bacterial cell processes. miniTurbo-based proximity labeling has emerged as a powerful tool for the identification of in vivo protein networks in bacteria. In this chapter, we outline a standardized protocol for implementing miniTurbo-dependent proximity labeling in bacterial models. This approach enables the unbiased identification of interacting and proximal proteins under native conditions, providing valuable insights into bacterial cell physiology.