Torenia bicolor is a highly valuable model plant for advancing transgenic research in ornamental plant development, particularly in the study of desirable traits such as flower color, morphology, and lifespan. Its unique suitability for genetic studies stems from its accessible and efficient transformation system, which outperforms many other ornamental species and enables precise modifications for trait improvement. Moreover, Torenia bicolor’s distinct floral structure, characterized by a prominently protruding embryo sac, offers an unparalleled model for exploring plant fertilization, pollen-pistil interactions, and reproductive biology, making it a critical resource for botanical research. The long-term preservation of Torenia bicolor germplasm through cryopreservation is essential to maintain genetic material for ongoing and future research. Cryopreservation, an ultra-low-temperature storage technique, ensures the viability of genetic resources by preventing genetic degradation over time. This approach is particularly valuable for conserving species with high scientific and horticultural importance, as well as those at risk of endangerment, thereby securing their genetic diversity and availability for subsequent breeding and transgenic studies.