Millingtonia hortensis L. contains many high-value volatiles (L-linalool) with medicinal properties in its flowers, leaves, and bark, such as the ability to increase the immune system and treat a variety of ailments, including those with antimicrobial properties. Pollen contributes significantly to the genetic makeup of plants. Thus, pollen cryopreservation is an important way to conserve the genetic diversity of M. hortensis L. Cryopreserved pollen provides scope for gene conservation at the haploid stage in M. hortensis L. Conservation of the haploid gene pool can be accomplished by consolidating and cryostoring pollen of the different crop species. This haploid gene pool can be a major source of useful genes for pre-breeding, genetic amelioration, hybridization, biotechnology, and other basic studies. Such a protocol, made available to breeders, would help to improve their breeding efficiency in terms of identifying potential pollen parents and preserving them to fulfill commercial requirements. The ultimate aim of pollen storage has been to establish a “Pollen Cryobank” through which pollen can be consolidated and obtained for breeding without any seasonal or geographic constraints.