Hymenocallis, known for their striking and fragrant flowers, are prized for ornamental purposes in gardens and floral arrangements. With their unique, spider-like flower shape, these plants serve as an intriguing subject for pollen viability research. This protocol presents a step-by-step approach for evaluating pollen viability in Hymenocallis speciosa, utilizing dehydration followed by assessment with the hanging drop technique using B&K germination medium. Thawed pollen is incubated in a moisture chamber for optimal germination, and viable pollen samples are cryopreserved in liquid nitrogen for long-term storage. The goal of this study is to refine pollen storage techniques and evaluate viability, providing valuable insights into conservation, plant breeding, and horticultural practices.