Protocol for the Pollen Cryopreservation of Clitoria ternatea L.
摘要
Clitoria ternatea, also called Asian pigeonwings or butterfly pea, is a twining evergreen legume known for its beautiful flowers and medicinal uses. It is grown for its ornamental appeal as well as being a forage legume and a cover crop. The plant contains bioactive compounds such as flavonoids and anthocyanins, which may have medicinal benefits, including anti-inflammatory and antioxidant effects. Additionally, it helps improve soil fertility by fixing nitrogen, making it valuable for sustainable farming. This plant grows well in different climates, temperatures, and altitudes, which allows it to be cultivated in various regions. However, preserving C. ternatea diversity long term can be difficult. Cryopreservation offers an effective solution by storing pollen at extremely low temperatures, keeping it viable for long periods and ensuring its availability at all times. This method is especially useful for C. ternatea, as it helps conserve its genetic material for future use in research, breeding, and cultivation. Cryopreservation helps protect the plant’s genetic resources from threats such as climate change, habitat loss, and over-harvesting. By using this technique, we can ensure that C. ternatea remains available for agricultural and medicinal purposes.