Barleria obtusa, also known as the Tropical Bush Violet, is a shrub found in tropical areas of Asia and Africa. The plant is used in traditional medicine to treat bronchitis and cough, and its chemicals have been found to have anti-inflammatory, antibacterial, and other healing properties. Although B. obtusa has many uses, its long-term preservation, especially its pollen, has not been studied much. Cryopreservation, which involves freezing plant material, is an important method for keeping species safe for the future. Some studies have already explored cryopreserving other parts of Barleria species, such as shoot tips, but there is no known method for preserving B. obtusa pollen. Optimizing a protocol for pollen cryopreservation will help to assist in its breeding practices and ensure its long-term genetic preservation. This chapter deals with how to improve the pollen cryopreservation process for B. obtusa, focusing on factors such as drying time and the optimized pollen germination medium to keep the plant viable for future conservation and breeding projects.