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Soybean, rich in protein and oil, is one of the most important oil crops globally. Currently, artificial hybridization between varieties remains the primary method for soybean breeding, which requires synchronized flowering times of the parent plants. Pollen cryopreservation offers an effective solution to overcome seasonal, geographical, and physiological barriers in hybridization and serves as a safe method for the long-term storage of plant germplasm resources. Standardizing in vitro pollen germination and cryopreservation techniques for soybean is therefore essential. This chapter outlines the methods for collecting and cryopreserving soybean pollen and the techniques for evaluating pollen viability before and after cryopreservation. The protocol presented aims to enhance the efficiency of soybean breeding and reduce the need to maintain plants in the field for regular pollen supply.

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Pollen Cryopreservation Protocol for Soybean (Glycine max (L.))

  • 实验方案

  • 发表日期：

    2025年

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  • Xiaona Liu,
  • Peiguo Wang,
  • Lingshi Xia,
  • Weiliang Pan,
  • Xin Liang,
  • Hongchang Jia,
  • Jinlu Tao,
  • Mahmoud Naser,
  • Shi Sun,
  • Tianfu Han

摘要

Soybean, rich in protein and oil, is one of the most important oil crops globally. Currently, artificial hybridization between varieties remains the primary method for soybean breeding, which requires synchronized flowering times of the parent plants. Pollen cryopreservation offers an effective solution to overcome seasonal, geographical, and physiological barriers in hybridization and serves as a safe method for the long-term storage of plant germplasm resources. Standardizing in vitro pollen germination and cryopreservation techniques for soybean is therefore essential. This chapter outlines the methods for collecting and cryopreserving soybean pollen and the techniques for evaluating pollen viability before and after cryopreservation. The protocol presented aims to enhance the efficiency of soybean breeding and reduce the need to maintain plants in the field for regular pollen supply.