Averrhoa carambola (starfruit), a member of the Oxalidaceae family, is an economically and ecologically significant species widely cultivated in tropical and subtropical regions, particularly in Southeast Asia. Beyond its culinary and medicinal applications, A. carambola plays a crucial role in biodiversity conservation. As a dioecious plant, it relies on cross-pollination, primarily facilitated by insect pollinators such as honeybees. The fruit is highly valued for its rich nutritional profile, containing antioxidants, vitamins, and minerals, and has been traditionally used for treating various health conditions. Pollen cryopreservation of A. carambola is a vital strategy for preserving genetic diversity and supporting breeding programs. By storing pollen at ultra-low temperatures, this method ensures long-term conservation of genetic material, providing a valuable resource for future plant breeding and biodiversity preservation efforts. Freshly collected pollen undergoes pretreatment steps, such as dehydration, before cryogenic storage. Experimental findings indicate that optimized cryopreservation protocols significantly enhance the viability of A. carambola pollen after thawing, proving the effectiveness of this method for genetic preservation. These findings outline the potential of cryopreservation as a critical strategy for conserving and utilizing the genetic resources of star fruit in breeding programs and research initiatives.