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Cryogenic electron microscopy (cryo-EMCryogenic electron microscopy (Cryo-EM)) has been an important technique for determining the structures of macromolecules at near-atomic resolution in their native states. In recent years, the structures of some respiratory syncytial virus (RSV) protein complexes, such as the RSV polymerase (L:P complex) and nucleoprotein-RNA complex, have been determined by cryo-EMCryogenic electron microscopy (Cryo-EM), providing insights into the mechanisms of viral RNA synthesis and facilitating antiviral development. Here, we describe the step-by-step protocols for the negative-stain and cryo-EMCryogenic electron microscopy (Cryo-EM) analysis of the RSV protein complexes.

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Single-Particle Electron Microscopy Analysis of Respiratory Syncytial Virus Protein Complexes

  • 实验方案

  • 发表日期：

    2025年

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  • Dongdong Cao,
  • Yunrong Gao,
  • Zhenhang Chen,
  • Natalie Horgan,
  • Bo Liang

摘要

Cryogenic electron microscopy (cryo-EMCryogenic electron microscopy (Cryo-EM)) has been an important technique for determining the structures of macromolecules at near-atomic resolution in their native states. In recent years, the structures of some respiratory syncytial virus (RSV) protein complexes, such as the RSV polymerase (L:P complex) and nucleoprotein-RNA complex, have been determined by cryo-EMCryogenic electron microscopy (Cryo-EM), providing insights into the mechanisms of viral RNA synthesis and facilitating antiviral development. Here, we describe the step-by-step protocols for the negative-stain and cryo-EMCryogenic electron microscopy (Cryo-EM) analysis of the RSV protein complexes.