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Fluorescence in situ hybridization (FISH) to visualize DNA or chromosomes in cells is well recognized as a valuable and versatile technique. The recent development of Oligopaint FISH is particularly useful for studies of chromosome structure, function, and evolution. Here, we describe a series of protocols for applying the Oligopaint FISH technique to mouse oocytes, eggs, and early-stage embryos. We describe techniques to adhere these cell types to microscopy slides, improving the ease of applying the FISH technique. We also provide an option to combine this with immunofluorescence (IF).

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Whole Mount Oligopaint Fluorescence In Situ Hybridization of Mouse Oocytes, Eggs, or Early-Stage Embryos

  • 实验方案

  • 发表日期：

    2025年

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  • Frances E. Clark,
  • Duilio M. Z. A. Silva,
  • Morgan Skinner,
  • Leah F. Rosin,
  • Naomi L. Greenberg,
  • Takashi Akera

摘要

Fluorescence in situ hybridization (FISH) to visualize DNA or chromosomes in cells is well recognized as a valuable and versatile technique. The recent development of Oligopaint FISH is particularly useful for studies of chromosome structure, function, and evolution. Here, we describe a series of protocols for applying the Oligopaint FISH technique to mouse oocytes, eggs, and early-stage embryos. We describe techniques to adhere these cell types to microscopy slides, improving the ease of applying the FISH technique. We also provide an option to combine this with immunofluorescence (IF).