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Traditional Western blot technology coupled with two-dimensional  SDS-PAGE has been used for decades to detect specific proteins in extracts. Here we describe an automated Western technology based on protein separation by capillary assay. Only 15 mouse oocytes are sufficient to obtain a quantitative protein signal in the assay we used here. This automated process is a good alternative to classical western blotting, as it is less time consuming, highly reproducible and requires less material.

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“JessTM Simple WesternTM”: A New Technology to Assess Protein Abundance in Mouse Oocytes

  • 实验方案

  • 发表日期：

    2025年

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  • Damien Cladière,
  • Katja Wassmann,
  • Sandra A. Touati

摘要

Traditional Western blot technology coupled with two-dimensional  SDS-PAGE has been used for decades to detect specific proteins in extracts. Here we describe an automated Western technology based on protein separation by capillary assay. Only 15 mouse oocytes are sufficient to obtain a quantitative protein signal in the assay we used here. This automated process is a good alternative to classical western blotting, as it is less time consuming, highly reproducible and requires less material.